Anti-6X His tag® 抗体 [3H2201] (Biotin) (ab15145)

製品の概要

  • 製品名Anti-6X His tag® antibody [3H2201] (Biotin)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3H2201] to 6X His tag® (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    In Western Blot of bacterial extracts, the antibody has been shown not to crossreact with any endogenous products. Some Reactivity may be seen with extracts of insect or mammalian cells.
  • 免疫原

    His-His-His-His-His-His conjugated to KLH.

  • 特記事項HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名3H2201
  • ミエローマNS1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.
ELISA 1/500 - 1/1000.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [3H2201] (Biotin) (ab15145) 使用論文

ab15145 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply message. I am sorry to confirm that the biotin binding of ab15145 has not been quantified, but it is typically in the range of 4-6 biotin molecules / antibody. The biotin is linked through lysine residues on the antibody s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"