Anti-6X His tag® 抗体 [33D10.D2] (Biotin) (ab106261)

製品の概要

  • 製品名
    Anti-6X His tag® antibody [33D10.D2] (Biotin)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin)
  • 標識
    Biotin
  • 特異性
    ab106261 detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino or carboxy-termini of targeted proteins in transfected or transformed cells.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 免疫原

    6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

  • 特記事項

    10-20 BAC molecules per Mouse Monoclonal Antibody

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    33D10.D2
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
ELISA 1/200000.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

プロトコール

参考文献

ab106261 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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