Application
Immunoprecipitation
Sample
Human Cell lysate - other (PDCD5 Purified from E Coli mixed with RRL)
Total protein in input
100 µg
Specification
PDCD5 Purified from E Coli mixed with RRL
Immuno-precipitation step
Protein A/G
Other product details
Dilution
1/100
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS with 1% NP40 and protease inhibitors
Western Blot antibody
Name
Non-Abcam antibody was used: mouse flag primary ab
Dilution
1/2000
Additional data
Additional Notes
Lane 1 MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa). Lanes 2-5 IP of his/flag tagged PDCD5 using ab18184 HIS antibody from Abcam from mixture of ~50 ng purified his/flag tagged PDCD5 and 15 uL RRL in 300 uL buffer. Lanes 6 and 7, purified PDCD5 from E. coli with ~60-70 ng protein. The Lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa). WB is a mouse flag primary ab with a goat anti-mouse secondary conjugated to IRDye800CW from Licor
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Chris Tracy
Verified customer
投稿 Dec 19 2012