Application
Flow Cytometry
Sample
Mouse Cell (BALB-3T3)
Specification
BALB-3T3
Preparation
Cell harvesting/tissue preparation method: trypsin/EDTA
Sample buffer: PBS with 0.1% BSA
Sample buffer: PBS with 0.1% BSA
Fixation
none
Permeabilization
No
Gating Strategy
Forward scatter and side scatter
Other product details
Dilution
1/15
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS with 0.1% BSA
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
BALB-3T3 cells, which express moderate amounts of EGFR, were incubated with a His6-tagged anti-EGFR fibronectin domain. The labeled cells were washed and incubated with 50 microliters of 1:15 diluted PE-conjugated anti-His6 antibody. Cells were washed and analyzed by flow cytometry. A control without fibronectin domain was also analyzed.
Image: Dashed black line is control without fibronectin. Solid red line is sample with His6-tagged anti-EGFR fibronectin domain.
Image: Dashed black line is control without fibronectin. Solid red line is sample with His6-tagged anti-EGFR fibronectin domain.
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投稿 May 09 2011