Anti-68kDa Neurofilament 抗体 (ab72997)

製品の概要

  • 製品名Anti-68kDa Neurofilament antibody
    68kDa Neurofilament 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to 68kDa Neurofilament
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, ICC/IF, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human
  • 免疫原

    Preparation of bovine spinal cord intermediate filaments.

  • ポジティブ・コントロール
    • Rat cerebral cortex lysate. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
ICC/IF 1/5000.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • 関連疾患Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • 配列類似性Belongs to the intermediate filament family.
  • ドメインThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • 翻訳後修飾O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 68 kDa neurofilament protein antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

Anti-68kDa Neurofilament antibody 画像

  • Anti-68kDa Neurofilament antibody (ab72997) at 1/10000 dilution + Rat cerebral cortex lysate

    Predicted band size : 61 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
  • ab72997 staining 68kDa Neurofilament in Mouse retina tissue by Immunohistochemistry (Frozen sections). Sections were fixed with formaldehyde and permeabilized in 0.3% Triton X-100 prior to blocking in 3% BSA for 1 hour at 25°C. ab72997 was diluted 1/500 and incubated with the sample for 4 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-Chicken antibody, diluted 1/500 was used as the secondary antibody.

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  • ab72997 staining 68kDa Neurofilament in rat 33B cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An AlexaFluor®555-conjugated goat anti-chicken polyclonal (1/1000) was used as the secondary antibody.

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  • ICC/IF image of ab72997 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72997 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab72997 staining 68kDa Neurofilament in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde for 1 hour and blocked with 10% goat serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated goat anti-chicken IgY polyclonal (1/1000) was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.

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Anti-68kDa Neurofilament antibody (ab72997) 使用論文

ab72997 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Mouse Tissue sections (Retina)
Specification Retina
Permeabilization Yes - 0.3% Triton X
Fixative Paraformaldehyde
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投稿 Aug 19 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Kelly)
Specification Kelly
Permeabilization No
Fixative Paraformaldehyde
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投稿 May 15 2014

Application Immunohistochemistry (Frozen sections)
Blocking step (agent) for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
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投稿 May 15 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Rat Cell (33B)
Specification 33B
Permeabilization No
Fixative Paraformaldehyde
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投稿 Apr 22 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Cell (Neuro2a)
Specification Neuro2a
Permeabilization No
Fixative Paraformaldehyde
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投稿 Apr 22 2014


The concentration of your lot (GR124671-2) of ab72997 is 21.5 mg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain lysate)
Loading amount 25 µg
Specification Mouse brain lysate
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Oct 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kelly Neuroblastoma cell line)
Specification Kelly Neuroblastoma cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (33B)
Specification 33B
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Aug 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neuro2a)
Specification Neuro2a
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Jun 07 2012

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