Anti-67kDa Laminin Receptor 抗体 [MLuC5] (ab3099)

製品の概要

  • 製品名Anti-67kDa Laminin Receptor antibody [MLuC5]
    67kDa Laminin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MLuC5] to 67kDa Laminin Receptor
  • アプリケーション適用あり: Inhibition Assay, IHC-Fr, WB, Flow Cyt, IHC-P, ICC/IFmore details
    適用なし: IHC-FoFr
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Small cell lung carcinoma N592 live cells.

  • ポジティブ・コントロール
    • Breast carcinoma.
  • 特記事項Molecular weight of Laminin Receptor = ~ 67 kDa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use at an assay dependent concentration. This antibody partially inhibits the binding of soluble Laminin (Martignone S, et. al).
IHC-Fr 1/200.
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
Flow Cyt Use at an assay dependent concentration.

ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • 追加情報Is unsuitable for IHC-FoFr.
  • ターゲット情報

    • 機能Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
    • 配列類似性Belongs to the ribosomal protein S2P family.
    • 翻訳後修飾Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
      Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
    • 細胞内局在Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 34/67 kDa laminin receptor antibody
      • 37 kDa laminin receptor precursor antibody
      • 37/67 kDa laminin receptor antibody
      • 37LRP antibody
      • 40S ribosomal protein SA antibody
      • 67 kDa laminin receptor antibody
      • 67LR antibody
      • Colon carcinoma laminin binding protein antibody
      • Colon carcinoma laminin-binding protein antibody
      • LAMBR antibody
      • Laminin receptor 1 antibody
      • Laminin-binding protein precursor p40 antibody
      • LAMR 1 antibody
      • LamR antibody
      • LAMR1 antibody
      • LBP antibody
      • LBP/p40 antibody
      • LRP antibody
      • LRP/LR antibody
      • Multidrug resistance associated protein MGr1 Ag antibody
      • Multidrug resistance associated protein MGr1Ag antibody
      • Multidrug resistance-associated protein MGr1-Ag antibody
      • NEM/1CHD4 antibody
      • p40 antibody
      • Ribosomal Protein SA antibody
      • rpsA antibody
      • RSSA_HUMAN antibody
      • SA antibody
      see all

    Anti-67kDa Laminin Receptor antibody [MLuC5] 画像

    • ICC/IF image of ab3099 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3099, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099) at 1/1000 dilution + Mouse pancreatic cancer tissue lysate at 35 µg

      Secondary
      HRP-conjugated Sheep anti-Mouse polyclonal at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 33 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      This image is courtesy of an anonymous Abreview.

      See Abreview

    • Overlay histogram showing MCF7 cells stained with ab3099 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3099, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099) 使用論文

    This product has been referenced in:
    • Conway MJ  et al. Mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. J Virol 88:164-75 (2014). Read more (PubMed: 24131723) »
    • Santilli G  et al. Polyphenol E enhances the antitumor immune response in neuroblastoma by inactivating myeloid suppressor cells. Clin Cancer Res 19:1116-25 (2013). Mouse . Read more (PubMed: 23322899) »

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Sample Xenopus laevis Tissue lysate - whole (brain)
    Gel Running Conditions Reduced Denaturing (12 % GEL)
    Loading amount 20 µg
    Specification brain
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    Verified customer

    投稿 Oct 27 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (colon carcinoma)
    Loading amount 10 µg
    Specification colon carcinoma
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Apr 05 2013

    Thank you for your phone enquiry regarding ab3099.

    I am very sorry for the delayed response.
    This antibody is sourced and unfortunately we have not testedit in inhibition assay in-house. According to this published paper, ab3099 (clone M...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (pancreatic cancer)
    Loading amount 35 µg
    Specification pancreatic cancer
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Dec 09 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (pancreatic tumor)
    Specification pancreatic tumor
    Fixative Acetone
    Permeabilization No
    Blocking step MOM kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Dec 04 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (pancreatic cancer)
    Specification pancreatic cancer
    Fixative Acetone
    Permeabilization No
    Blocking step MOM kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Dec 03 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (keratinocytes)
    Specification keratinocytes
    Fixative Formaldehyde
    Permeabilization Yes - PBST
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Oct 04 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (MCF-7 Cells (human breast adenocarcinoma))
    Specification MCF-7 Cells (human breast adenocarcinoma)
    Fixative Formaldehyde
    Permeabilization Yes - PBST
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

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    Verified customer

    投稿 Oct 01 2010

    Thank you for your enquiry. I have been in contact with the source of this antibody and I have determined that this antibody was used by FACS in the following publication: Martignone S et al. Characterization of two monoclonal antibodies directe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"