Anti-67kDa Laminin Receptor 抗体 (ab137388)
Key features and details
- Rabbit polyclonal to 67kDa Laminin Receptor
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-67kDa Laminin Receptor antibody
67kDa Laminin Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 67kDa Laminin Receptor -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Monkey -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-294 of Human 67kDa Laminin Receptor (Uniprot ID: P08865).
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells; Human CA922 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137388の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
ターゲット情報
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機能
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. -
配列類似性
Belongs to the ribosomal protein S2P family. -
翻訳後修飾
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions. -
細胞内局在
Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281898 Cow
- Entrez Gene: 3921 Human
- Entrez Gene: 100505031 Mouse
- Entrez Gene: 16785 Mouse
- Entrez Gene: 641351 Pig
- Entrez Gene: 29236 Rat
- Omim: 150370 Human
- SwissProt: P26452 Cow
see all -
別名
- 34/67 kDa laminin receptor antibody
- 37 kDa laminin receptor precursor antibody
- 37/67 kDa laminin receptor antibody
see all
画像
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Anti-67kDa Laminin Receptor antibody (ab137388) at 1/1000 dilution + HepG2 whole cell lysate
at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Anti-67kDa Laminin Receptor antibody (ab137388) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labelling 67kDa Laminin Receptor with ab137388 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded Human CA922 xenograft tissue, labelling 67kDa Laminin Receptor with ab137388 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab137388 は 5 報の論文で使用されています。
- Della Via FI et al. (-)-Epigallocatechin-3-gallate induces apoptosis and differentiation in leukaemia by targeting reactive oxygen species and PIN1. Sci Rep 11:9103 (2021). PubMed: 33907248
- Jiang X et al. Control of ribosomal protein synthesis by the Microprocessor complex. Sci Signal 14:N/A (2021). PubMed: 33622983
- Garita-Hernandez M et al. AAV-Mediated Gene Delivery to 3D Retinal Organoids Derived from Human Induced Pluripotent Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32028585
- Ashok A et al. Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma. Sci Rep 9:13090 (2019). PubMed: 31511544
- Khajuria RK et al. Ribosome Levels Selectively Regulate Translation and Lineage Commitment in Human Hematopoiesis. Cell 173:90-103.e19 (2018). PubMed: 29551269