製品の概要

  • 製品名
    Anti-5HT4 Receptor antibody
    5HT4 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 5HT4 Receptor
  • 特異性
    5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein.
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
  • 免疫原

    Synthetic peptide: LMAILGNLLV MVAVCWDRQ L RKIKTNYF IV SLAFADL LVS, corresponding to amino acids 31/70 of Human 5HT4 Receptor

  • ポジティブ・コントロール
    • 293 cells, treated with insulin (0.01U/ml, 15mins)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

ターゲット情報

  • 関連性
    The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression.
  • 細胞内局在
    Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
  • 参照データベース
  • 別名
    • 5 HT 4 antibody
    • 5 HT4 antibody
    • 5 HT4R antibody
    • 5 hydroxytryptamine (serotonin) receptor 4, G protein coupled antibody
    • 5 hydroxytryptamine 4 receptor antibody
    • 5 hydroxytryptamine receptor 4 antibody
    • 5 hydroxytryptamine serotonin receptor 4 antibody
    • 5 hydroxytryptamine4 receptor antibody
    • 5-HT-4 antibody
    • 5-ht4 antibody
    • 5-HT4R antibody
    • 5H4 antibody
    • 5HT 4 antibody
    • 5HT4 antibody
    • 5ht4 receptor antibody
    • 5HT4R antibody
    • Cardiac 5 HT4 receptor antibody
    • HTR 4 antibody
    • HTR4 antibody
    • serotonin 4 receptor antibody
    • Serotonin 5 HT4 receptor antibody
    • serotonin 5-ht-4 receptor antibody
    • serotonin 5-ht-4a antibody
    • Serotonin receptor 4 antibody
    see all

画像

  • All lanes : Anti-5HT4 Receptor antibody (ab60359)

    Lane 1 : 293 cells treated with insulin (0.01U/ml, 15 mins)
    Lane 2 : 293 cells treated with insulin (0.01U/ml, 15 mins) with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 44 kDa
  • ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right).
  • All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

I am waiting for replies from some of the laboratories that produced these antibodies.

Of the antibodies for which we know the immunogen sequence (others are considered proprietary by the laboratories that produce the antibodies), the only t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human adrenal, brain, lung)
Specification
human adrenal, brain, lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
No
Blocking step
Ab94737 kit blocking agent as blocking agent for 10 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
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投稿 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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