製品の概要

  • 製品名Anti-5HT3B receptor antibody
    5HT3B receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 5HT3B receptor
  • 特異性Specific to 5HT3B - does not recognise 5HT3A receptor This antibody recognizes the intracellular region of the 5HT3B subunit.
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 320-338 of Human 5HT3B receptor (large intracellular loop region)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • 組織特異性Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta.
  • 配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily.
  • 翻訳後修飾N-glycosylation required for membrane localization.
  • 細胞内局在Cell membrane. Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 HT3 B antibody
    • 5 HT3B antibody
    • 5 HTR3B antibody
    • 5 hydroxytryptamine 3 receptor B subunit antibody
    • 5 hydroxytryptamine receptor 3B antibody
    • 5 hydroxytryptamine serotonin receptor 3B antibody
    • 5-HT3-B antibody
    • 5-HT3B antibody
    • 5-hydroxytryptamine receptor 3B antibody
    • 5HT3B_HUMAN antibody
    • 5HTR3B antibody
    • Htr3b antibody
    • Serotonin gated ion channel subunit antibody
    • Serotonin receptor 3B antibody
    see all

Anti-5HT3B receptor antibody 画像

  • All lanes : Anti-5HT3B receptor antibody (ab39629) at 1/500 dilution

    Lane 1 : Lysate from HEK 293 cells
    Lane 2 : Lysate from HEK 293 cells transfected with 5HT3A receptor
    Lane 3 : Lysate from HEK 293 cells transfected with 5HT3A receptor and 5HT3B receptor
    Lane 4 : Human hippocampus lysate


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • HEK 293 cells transfected with 5HT3B receptor were stained with ab39629 diluted 1/500 and anti-rabbit secondary antibody conjugated to Alexa Fluor 488 diluted 1/1000(green). Nuclei are labelled with Hoescht 33258 (blue).
  • Immunohistochemistry of human hippocampus stained with ab39269 diluted 1/500. Immunoreactivity with 5HT3B receptor can be seen in (i)CA1 field, (ii)CA2, (iii)CA3 and (iv)CA4/dentate gyrus.

Anti-5HT3B receptor antibody (ab39629) 使用論文

This product has been referenced in:

See 1 Publication for this product

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Thank you very much for your interest in ab39629.
To our knowledge, ab39629 has not been tested in flow cytometry. Therefore, I can offer a discount off a future purchase if you buy ab39629 now, test it in flow cytometry and submit feedback to us ...

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Thank you for contacting us. We would normally recommend using a rabbit IgG and use the same donkey anti-rabbit FITC secondary in order to detect any interaction. Your Rabbit IgG Isotype control-FITC should be applicable but may cause some differenc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"