Anti-5HT3A receptor/5ht3 抗体 (ab51950)
Key features and details
- Goat polyclonal to 5HT3A receptor/5ht3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-5HT3A receptor/5ht3 antibody
5HT3A receptor/5ht3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to 5HT3A receptor/5ht3 -
由来種
Goat -
特異性
ab51950 is expected to recognise both reported isoforms (NP_998786.1 and NP_000860.1). -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human 5HT3A receptor/5ht3 aa 390-401 (internal sequence).
Sequence:CGPQDFEKSPRDR
Database link: P46098 -
ポジティブ・コントロール
- Flow Cyt (Intra): HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.05% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab51950 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51950の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. -
組織特異性
Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. -
細胞内局在
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3359 Human
- Omim: 182139 Human
- SwissProt: P46098 Human
- Unigene: 413899 Human
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別名
- 5 HT 3 antibody
- 5 HT3 receptor antibody
- 5 HT3A antibody
see all
画像
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Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labelling 5HT3A receptor with ab51950. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab51950 は 2 報の論文で使用されています。
- Yamada J et al. PSA-NCAM Colocalized with Cholecystokinin-Expressing Cells in the Hippocampus Is Involved in Mediating Antidepressant Efficacy. J Neurosci 40:825-842 (2020). PubMed: 31801810
- Li F et al. Shuyu Capsules Relieve Premenstrual Syndrome Depression by Reducing 5-HT3AR and 5-HT3BR Expression in the Rat Brain. Neural Plast 2016:7950781 (2016). PubMed: 27725889