製品の概要

  • 製品名Anti-5HT3A receptor antibody
    5HT3A receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 5HT3A receptor
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Rat 5HT3A receptor. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
    Database link: P35563

  • ポジティブ・コントロール
    • human brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.

ターゲット情報

  • 機能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • 組織特異性Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
  • 配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
  • 細胞内局在Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3RA antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR3 antibody
    • Htr3a antibody
    • Serotonin receptor 3A antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all

Anti-5HT3A receptor antibody 画像

  • All lanes : Anti-5HT3A receptor antibody (ab13897) at 3 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : Mouse Brain tissue lysate
    Lane 3 : Rat Brain tissue lysate


    Predicted band size : 55 kDa
  • IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-5HT3A receptor antibody (ab13897) 使用論文

This product has been referenced in:
  • Nguyen H  et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil 20:228-35 (2008). WB ; Rat . Read more (PubMed: 17919312) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Cell (SH-SY5Y))
Permeabilization Yes - TRITON X-100 (0'25%)
Specification Human Cell (SH-SY5Y)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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投稿 Dec 06 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Tissue (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
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投稿 Sep 25 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10% SDS-acryamide gel)
Sample Rat Tissue lysate - whole (rat-colon)
Specification rat-colon
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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投稿 Sep 22 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Mouse Tissue sections (tongue, taste buds)
Specification tongue, taste buds
Permeabilization Yes - 0.3 % Triton, and 1 % Triton
Fixative Paraformaldehyde
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投稿 May 02 2014

Thank you for contacting Abcam.

The amino acids correspond to the protein sequence with the signal peptide (SwissProt: P35563). We normally look for at least 85% degree of homology before we would recommend using an antibody in an untested sp...

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Thank you for contacting us. The detection kit we used for the IHC image is the Leica Bond refine polymer detection kit (for the automated Leica Bond IHC system). The system uses small linkers to enhance and refine the detection of the target p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat cerebellar homogenate)
Loading amount 20 µg
Specification rat cerebellar homogenate
Treatment control and 20 mg/kg cocaine
Gel Running Conditions Reduced Denaturing
Blocking step Commercially available blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
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Mr. Jean-Bernard Dietrich

Verified customer

投稿 Sep 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"