Anti-5HT2C Receptor 抗体 (ab32887)

製品の概要

  • 製品名
    Anti-5HT2C Receptor antibody
    5HT2C Receptor 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to 5HT2C Receptor
  • 特異性
    Ab32887 recognises 5HT2C Receptor.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-QVENLELPVN

    , corresponding to amino acids 437-446 of Human 5HT2C Receptor.

  • ポジティブ・コントロール
    • Lysates of EBV immortalised Lymphoblastoids, T cell, MOLT4 and monocytic cell line U937.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
ELISA 1/64000.
IHC-Fr Use at an assay dependent concentration. Abreview 30686 suggests that this antibody is not suitable for this application

ターゲット情報

  • 機能
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • ドメイン
    The PDZ domain-binding motif is involved in the interaction with MPDZ.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 Hydroxytryptamine 2C receptor antibody
    • 5-HT-1C antibody
    • 5-ht-1c receptor antibody
    • 5-HT-2C antibody
    • 5-HT1C antibody
    • 5-HT2C antibody
    • 5-HTR2C antibody
    • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
    • 5-hydroxytryptamine receptor 1C antibody
    • 5-hydroxytryptamine receptor 2C antibody
    • 5HT1C antibody
    • 5HT2C antibody
    • 5HT2C_HUMAN antibody
    • 5HTR2C antibody
    • 5Hydroxytryptamine 2C receptor antibody
    • Htr1c antibody
    • HTR2C antibody
    • serotonin 1c receptor antibody
    • serotonin 2c receptor antibody
    • Serotonin 5-HT-2C receptor antibody
    • Serotonin receptor 2C antibody
    see all

Anti-5HT2C Receptor antibody 画像

  • Anti-5HT2C Receptor antibody (ab32887) at 1 µg/ml + EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-5HT2C Receptor antibody (ab32887) 使用論文

ab32887 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Great news! The blocking peptide has been added as 5HT2C Receptor peptide ab168550.

http://www.abcam.com/5ht2c-receptor-peptide-ab168550.html

I hope this information helps. Please contact us wit...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
formalin
Antigen retrieval step
Enzymatic
Permeabilization
Yes - 1xPBT
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C
Username

Abcam user community

Verified customer

投稿 Sep 04 2012


I am very pleased to hear you would like to accept our offer and test ab32887 and ab44640 in IHC-FoFr and WB, respectively. These codes will give you: 1 free primary antibody each before the expiration date. To redeem this offer, please submit an ...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (hypothalamus PVN sections)
Specification
hypothalamus PVN sections
Fixative
ethanol 70%
Permeabilization
Yes - triton-x
Blocking step
2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Ms. Rebecca Ring

Verified customer

投稿 Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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