製品の概要

  • 製品名Anti-5HT1A Receptor antibody
    5HT1A Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 5HT1A Receptor
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse, Chicken, Dog, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Rat 5HT1A Receptor aa 100-200 conjugated to keyhole limpet haemocyanin.
    Database link: P19327
    (Peptide available as ab98259)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Mouse and Rat hippocampus tissue lysate as well as the following whole cell lysates: Caco2; HepG2; Jurkat. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 46 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 HT 1A antibody
    • 5 HT receptor 1A antibody
    • 5 HT1A antibody
    • 5 HT1A receptor antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled antibody
    • 5 hydroxytryptamine receptor 1A antibody
    • 5-HT-1A antibody
    • 5-HT1A antibody
    • 5-hydroxytryptamine receptor 1A antibody
    • 5HT 1A antibody
    • 5HT 1A receptor antibody
    • 5HT receptor 1A antibody
    • 5HT1a antibody
    • 5HT1A_HUMAN antibody
    • ADRB2RL1 antibody
    • ADRBRL1 antibody
    • G 21 antibody
    • G protein coupled receptor antibody
    • G-21 antibody
    • Gpcr 18 antibody
    • Gpcr18 antibody
    • Guanine nucleotide binding regulatory protein-coupled receptor antibody
    • Htr1a antibody
    • PFMCD antibody
    • Serotonin 1A receptor antibody
    • Serotonin 5HT1A receptor antibody
    • Serotonin receptor 1A antibody
    see all

Anti-5HT1A Receptor antibody 画像

  • All lanes : Anti-5HT1A Receptor antibody (ab85615) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Rat Hippocampus Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab85615 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of 5HT1A Receptor staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85615, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-5HT1A Receptor antibody (ab85615) 使用論文

This product has been referenced in:
  • Zheng Y  et al. In silico Analysis and Experimental Validation of Lignan Extracts from Kadsura longipedunculata for Potential 5-HT1AR Agonists. PLoS One 10:e0130055 (2015). Read more (PubMed: 26076134) »

See 1 Publication for this product

Product Wall

I am waiting for replies from some of the laboratories that produced these antibodies.

Of the antibodies for which we know the immunogen sequence (others are considered proprietary by the laboratories that produce the antibodies), the only t...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

Verified customer

投稿 Jul 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"