Anti-5HT7 Receptor 抗体 - N-terminal (ab137493)
Key features and details
- Rabbit polyclonal to 5HT7 Receptor - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-5HT7 Receptor antibody - N-terminal
5HT7 Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 5HT7 Receptor - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to a region within N terminal amino acids 1-45 of Human 5HT7 Receptor (Uniprot ID: P34969).
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ポジティブ・コントロール
- A549, HeLa, Jurkat and NT2D1 whole cell lysates; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137493の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. -
組織特異性
Isoform A is the predominant isoform in spleen, caudate and hippocampus. Isoform B is expressed at lower levels. Isoform D is a minor isoform in term of expression. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3363 Human
- Entrez Gene: 15566 Mouse
- Entrez Gene: 65032 Rat
- Omim: 182137 Human
- SwissProt: P34969 Human
- SwissProt: P32304 Mouse
- SwissProt: P32305 Rat
- Unigene: 73739 Human
see all -
別名
- 5 HT 7 antibody
- 5 HT X antibody
- 5 hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase coupled) antibody
see all
画像
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Anti-5HT7 Receptor antibody - N-terminal (ab137493) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labelling 5HT7 Receptor with ab137493 at 1/200 dilution. The lower image is co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab137493 は 4 報の論文で使用されています。
- Chang WY et al. 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome. Lab Invest 102:1023-1037 (2022). PubMed: 35585132
- Romero-Reyes J et al. Differential localization of serotoninergic system elements in human amniotic epithelial cells†. Biol Reprod 105:439-448 (2021). PubMed: 34057176
- Sheng X et al. HTR7 promotes laryngeal cancer growth through PI3K/AKT pathway activation. Ann Transl Med 9:840 (2021). PubMed: 34164474
- Zhu H et al. MiRNA-29a modulates visceral hyperalgesia in irritable bowel syndrome by targeting HTR7. Biochem Biophys Res Commun 511:671-678 (2019). PubMed: 30827505