製品の概要

  • 製品名Anti-58K Golgi protein antibody
    58K Golgi protein 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to 58K Golgi protein
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide:

    SQLVECVPNFSEGKNQ

    , corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein.

  • ポジティブ・コントロール
    • Human liver lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.03 - 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 18315599

ターゲット情報

  • 機能Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • パスウェイAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • 関連疾患Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • 配列類似性In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Anti-58K Golgi protein antibody 画像

  • Anti-58K Golgi protein antibody (ab19072) at 0.03 µg/ml + tissue lysates prepared from human liver

    Secondary
    HRP-conjugated anti goat IgG

    Predicted band size : 59 kDa


    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab19072 at 3.8µg/ml staining 58K Golgi protein in human liver tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded tissue sections). Steamed antigen retrieval in citrate buffer pH 6 was performed. AP staining procedure was used for detection.

Anti-58K Golgi protein antibody (ab19072) 使用論文

This product has been referenced in:
  • Schrage YM  et al. Aberrant heparan sulfate proteoglycan localization, despite normal exostosin, in central chondrosarcoma. Am J Pathol 174:979-88 (2009). IHC-P ; Human . Read more (PubMed: 19179614) »
  • Willems SM  et al. Myxoid tumours of soft tissue: the so-called myxoid extracellular matrix is heterogeneous in composition. Histopathology 52:465-74 (2008). IHC-P ; Human . Read more (PubMed: 18315599) »

See all 3 Publications for this product

Product Wall

Thank you for send the question again. You can use the antibodies with the secondary, however be advised that we have only tested tissue sections and not cell cultures, though I would expect it is likely to work for cells as well. Please let me know if...

Read More

Unfortunately none of our Golgi markers have been tested against protists. Sorry I couldn't help you more,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"