製品の概要

  • 製品名
    Anti-53BP1 (phospho S25) antibody
    53BP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 53BP1 (phospho S25)
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Phosphorylated synthetic peptide corresponding to a portion of 53BP1 around serine 25 (NP_005648.1)

  • ポジティブ・コントロール
    • Human colon adenocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • 関連疾患
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • 配列類似性
    Contains 2 BRCT domains.
  • 翻訳後修飾
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 (phospho S25) antibody 画像

  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (mock phosphatase treated section), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (lambda and CIP phosphatase treated), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue,

プロトコール

Anti-53BP1 (phospho S25) antibody (ab82550) 使用論文

This product has been referenced in:
  • Lunardi A  et al. Suppression of CHK1 by ETS Family Members Promotes DNA Damage Response Bypass and Tumorigenesis. Cancer Discov 5:550-63 (2015). IHC ; Human . Read more (PubMed: 25653093) »
  • Roossink F  et al. The role of ATM and 53BP1 as predictive markers in cervical cancer. Int J Cancer 131:2056-66 (2012). IHC-P ; Human . Read more (PubMed: 22323184) »

See all 2 Publications for this product

Product Wall

These are very similar antibodies.

The ab82550 Anti-53BP1 (phospho S25) antibody is reformulated to specifically cater to needs expressed by IHC specialists to have antibodies that can be used between a 1:100 and 1:500 dilution. Both are teste...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録