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A phosphorylated synthetic peptide representing a portion of human 53BP1 around serine 25 (NP_005648.1.)
Our Abpromise guarantee covers the use of ab70323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa).|
|IP||Use at 1-4 µg/mg of lysate.|
|Flow Cyt||Use 0.25µg for 106 cells.
0.25 μg per 1 million cells in a 150 mcl reaction.
ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab70323 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"