製品の概要

  • 製品名
    Anti-53BP1 antibody
    53BP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 53BP1
  • 由来種
    Rabbit
  • 特異性
    This antibody is specific for 53BP1 protein.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide representing a portion of human 53BP1 encoded exon 19 (LocusLink ID 7158).

  • ポジティブ･コントロール
    • U205, 293T and MO59K cell lysates (human). MEF lysates (mouse).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
ICC/IF 1/200 - 1/1000.

ターゲット情報

  • 機能
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • 関連疾患
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • 配列類似性
    Contains 2 BRCT domains.
  • 翻訳後修飾
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 53 BP1 antibody
      • 53BP1 antibody
      • FLJ41424 antibody
      • MGC138366 antibody
      • p202 antibody
      • p53 binding protein 1 antibody
      • p53 BP1 antibody
      • p53-binding protein 1 antibody
      • p53BP1 antibody
      • TP53 BP1 antibody
      • TP53B_HUMAN antibody
      • Tp53bp1 antibody
      • TRP53 BP1 antibody
      • Tumor protein 53 binding protein 1 antibody
      • Tumor protein p53 binding protein 1 antibody
      • Tumor suppressor p53 binding protein 1 antibody
      • Tumor suppressor p53-binding protein 1 antibody
      see all

    画像

    • Western blot - 53BP1 antibody (ab36828)
      Western blot - 53BP1 antibody (ab36828)
      Anti-53BP1 antibody (ab36828) at 1/2000 dilution + Whole cell lysate from human U205 cells at 20 µg

      Developed using the ECL technique.

      Predicted band size: 214 kDa
      Observed band size: 220 kDa (why is the actual band size different from the predicted?)



      The arrow next to the image points to 53BP1 resolved on a 3 to 8% trisacetate gel.

    参考文献

    This product has been referenced in:
    • Han W  et al. Nitric oxide mediated DNA double strand breaks induced in proliferating bystander cells after alpha-particle irradiation. Mutat Res 684:81-9 (2010). ICC/IF ; Chinese hamster . Read more (PubMed: 20026341) »

    See 1 Publication for this product

    ab36828 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    Could you please tell me whether you sell an antibody which recognises
    specifically the tudor domain of the gene 53bp1 (ncbi gene id 7158). This
    would correspond to an epitope consisting of residues 495-534 in this gene.

    Thank you for your inquiry.


    I have checked our catalog regarding your search for an p53bpi antibody which binds in the
    tudor domain of this protein.

    Unfortunately, we have antibodies which will bind the N-or the C-Terminu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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