Anti-53BP1 抗体 (ab21083)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-53BP1 antibody
53BP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 53BP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human 53BP1 (C terminal). Conjugated to a carrier protein.
Database link: Q12888 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21083の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/3000. Detects a band of approximately 350 kDa (predicted molecular weight: 220 kDa).
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IHC-P |
1/1000 - 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/3000. Detects a band of approximately 350 kDa (predicted molecular weight: 220 kDa). |
IHC-P
1/1000 - 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
関連疾患
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
配列類似性
Contains 2 BRCT domains. -
翻訳後修飾
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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参照データベース
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
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別名
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 53BP1 with ab21083 at 1/4000 dilution. Nuclear staining is observed. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-53BP1 antibody (ab21083) at 1/500 dilution
Lane 1 : 53BP1 shRNA non-transfected HeLa whole cell extracts
Lane 2 : 53BP1 shRNA transfected HeLa whole cell extracts
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 220 kDa
Observed band size: 350 kDa why is the actual band size different from the predicted?5% SDS-PAGE
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All lanes : Anti-53BP1 antibody (ab21083) at 1/2000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lane 5 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 220 kDa
Observed band size: 350 kDa why is the actual band size different from the predicted?5% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (Nitrocellulose membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 1/2000, 4?, overnight.
Secondary antibody incubation: Rabbit IgG antibody (HRP), 1/10,000, RT, 1hr.
Washing condition: 5 ml TBST, 4 x 5min.
Exposure system: Trident plus Western HRP Substrate.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling 53BP1 with ab21083 at 1/4000 dilution. Nuclear staining is observed. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-53BP1 antibody (ab21083) at 1/500 dilution
Lane 1 : Neuro2A whole cell extracts
Lane 2 : C8D30 whole cell extracts
Lane 3 : NIH-3T3 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 220 kDa
Observed band size: 350 kDa why is the actual band size different from the predicted?5% SDS-PAGE
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ab21083 (2µg/ml) staining 53BP1 in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is ubiquitous nuclear staining throughout.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (101)
ab21083 は 101 報の論文で使用されています。
- Tirado-Class N et al. PHIP variants associated with Chung-Jansen syndrome disrupt replication fork stability and genome integrity. Cold Spring Harb Mol Case Stud 8:N/A (2022). PubMed: 35863899
- Feng YL et al. DNA nicks induce mutational signatures associated with BRCA1 deficiency. Nat Commun 13:4285 (2022). PubMed: 35879372
- Hu X et al. KMT2C-deficient tumors have elevated APOBEC mutagenesis and genomic instability in multiple cancers. NAR Cancer 4:zcac023 (2022). PubMed: 35898555
- Zhang Y et al. RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11. iScience 25:105115 (2022). PubMed: 36185355
- Paes Dias M et al. Loss of nuclear DNA ligase III reverts PARP inhibitor resistance in BRCA1/53BP1 double-deficient cells by exposing ssDNA gaps. Mol Cell 81:4692-4708.e9 (2021). PubMed: 34555355