Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were treated with bleomycin to induce DNA double strand breaks. They were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a strong nuclear focal staining pattern. MeOH-fixed samples were also evaluated a produced a similar staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Aug 20 2014