Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole embryos (~10 embryos per lane))
Loading amount
200000 cells
Specification
whole embryos (~10 embryos per lane)
Treatment
X-rays (0, 25 and 50 Gy)
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: thermo scientific
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Pierce super signal west pico
Exposure
30 minute(s) and 0 second(s)
Negative control
untreated sample
Additional data
Abcam response
Abcam does not recommend using ab70323 in Zebrafish as there is only 30% homology between the immunogen used to raise this antibody and the 53BP1 protein in this species. Abcam would like to recommend that researches interested in detecting 53BP1 (phospho S25) in Zebrafish would use a different antibody. Thank you for your valuable feedback, we hope this will be useful for other customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 20 2009