Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung bronchial biopsy)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung bronchial biopsy
Blocking step
20% FCS, 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
GMA-embedded
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: BIOTINYLATED ANTI-MOUSE IGG (H+L), MADE IN GOAT,
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 23 2018