Using a conjugated 5 Methoxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
(a) 5 Methoxytryptamine-G-PL concentration/unconjugated or conjugated indolealkylamine concentration at half displacement
(b) Non-reduced conjugate
G = Glutaraldehyde, PL = poly-Lysine
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry: 1/2000 - 1/5000. Tested using a PAP technique on free floating rat raphe nuclei.
ELISA: assay dependent.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
5-methoxytryptamine (5-MT) is a derivative of serotonin (or 5HT) and also a metabolite and precursor of melatonin. This potent serotonergic neuromodulator is involved in numerous physiological systems, e.g an article published in Pharmacol Biochem Behav. 2004 Feb;77(2):275-9 suggests a role melatoinin metabolites in nociception.