Anti-5 Lipoxygenase/5-LO (phospho S523) 抗体 (ab30573)
Key features and details
- Rabbit polyclonal to 5 Lipoxygenase/5-LO (phospho S523)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-5 Lipoxygenase/5-LO (phospho S523) antibody
5 Lipoxygenase/5-LO 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 5 Lipoxygenase/5-LO (phospho S523) -
由来種
Rabbit -
特異性
Specific for the ~80k 5 Lipoxygenase/5-LO phosphorylated at Ser523 in western blots of rat hippocampal lysates.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide within Human 5 Lipoxygenase/5-LO (phospho S523). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Rat cortex tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30573の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
The immunolabeling is completely eliminated by treatment with lambda-Phosphatase. |
特記事項 |
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WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa). The immunolabeling is completely eliminated by treatment with lambda-Phosphatase. |
ターゲット情報
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機能
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. -
パスウェイ
Lipid metabolism; leukotriene A4 biosynthesis. -
配列類似性
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
翻訳後修飾
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. -
細胞内局在
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association. - Information by UniProt
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参照データベース
- Entrez Gene: 25290 Rat
- SwissProt: P12527 Rat
- Unigene: 9662 Rat
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別名
- 5 Lipoxygenase antibody
- 5 LO antibody
- 5 LOX antibody
see all
画像
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All lanes : Anti-5 Lipoxygenase/5-LO (phospho S523) antibody (ab30573) at 1/1000 dilution
All lanes : rat cortex lysate
Predicted band size: 78 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Lane 2 was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5Lipoxygenase
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab30573 は 1 報の論文で使用されています。
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224