製品の概要

  • 製品名Anti-4 Hydroxynonenal antibody
    4 Hydroxynonenal 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 4 Hydroxynonenal
  • 特異性Specifically binds to HNE modified proteins.
  • アプリケーション適用あり: IHC-Fr, WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, a wide range of other species
  • 免疫原

    HNE conjugated to BC.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.0146% EDTA, 0.44% Sodium chloride, 50% Glycerol, 1.23% Sodium phosphate
  • Concentration information loading...
  • 特記事項(精製)This antibody was purified by an HNE modified Protein-Sepharose affinity column.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab46545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 24140865
WB Use at an assay dependent concentration. Can be blocked with Hydroxynonenal modified Bovine Serum Albumin (ab194193).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000 - 1/20000.

ターゲット情報

  • 関連性Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • 細胞内局在Cytoplasmic
  • 別名
    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody

Anti-4 Hydroxynonenal antibody 画像

  • Image from Wang J et al., PLoS One. 2013;8(1):e53951. Fig 7(A).; doi: 10.1371/journal.pone.0053951.

    Frozen mouse cardiac tissue was homogenized with lysis buffer containing 50 mmol/L Tris-HCl (pH7.5), 5 mmol/L EDTA, 10 mmol/L EGTA, 1X cock tail protease inhibitor, 1X alkaline phosphatase inhibitor and 1X acid phophatase inhibitor, 50 ug/ml phenylmenthysulfonyl fluoride and 1.23 mg/ml Chaps. Extracts were centrifuged at 12000 rpm at 4°C for 15 minutes. 10 ug of the sample proteins was mixed with loading buffer (40 mmol/L Tris-HCl, pH 6.8, 1% SDS, 50 mmol/L DTT, 7.5% glycerol and 0.003% bromophenol blue and heated at 95°C for 5 minutes, and subjected to electrophoresis on a gradient gel (4% to 12%) at 120V. After electrophoresis, the protein was transferred to a PVDF membrane in a transfer buffer. The PVDF membrane was rinsed briefly in TBS buffer containing 50 mM Tris, 137 mM NaCl, pH 7.5 and blocked in buffer (5% milk with 0.5% BSA in TBST buffer (TBS buffer containing 0.1% tween 20) at room temperature for 1 hour. The membrane was then incubated with rabbit anti 4-hydroxy-2-noneal (4HNE) antibody at 1/3000 dilution at 4°C over night, followed by washing three times. The secondary antibody was incubated with the membrane for another one hour at room temperature. Finally the antigen-antibody complexes were visualized with use of an enhanced chemiluminescence kit. Anti-GAPDH (Abcam) was used for normalizing.

     

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling 4-Hydroxynonenal with ab46545 at a dilution of 1/50 incubated for 13 hours at 4°C. Heat mediated antigen retrival was performed with Tris-EDTA at pH 9.0. Permeabilzation was via 0.025% Triton X-100. Blocking was with ab93695 ABC kit at 1% for 20 minutes at room temperature. ab93695 detection kit was used for signal amplification.

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  • Immunohistochemical analysis of Human uterine tissue with intrauterine growth restriction, staining 4 Hydroxynonenal with ab46545 at 1/200 dilution. Staining was detected using AEC.

Anti-4 Hydroxynonenal antibody (ab46545) 使用論文

This product has been referenced in:
  • Yu YH  et al. PKC-ALDH2 Pathway Plays a Novel Role in Adipocyte Differentiation. PLoS One 11:e0161993 (2016). WB, IP ; Mouse . Read more (PubMed: 27575855) »
  • Softic S  et al. Lipodystrophy Due to Adipose Tissue-Specific Insulin Receptor Knockout Results in Progressive NAFLD. Diabetes 65:2187-200 (2016). Read more (PubMed: 27207510) »

See all 36 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization No
Specification liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% normal buffered formalin
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投稿 May 08 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Permeabilization No
Specification heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Acetone
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投稿 Apr 17 2017

Application IHC - Wholemount
Sample Mouse Tissue (Intestine organoids)
Specification Intestine organoids
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投稿 Apr 06 2017

Application Western blot
Sample Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions Reduced Denaturing (12,5%)
Loading amount 70 µg
Specification Bone tissue (Tibia)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Sep 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization Yes - 0.025% Triton x-100
Specification Bone tissue (Femur)
Blocking step Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Sep 11 2015

Application Western blot
Sample Mouse Tissue lysate - whole (muscle tissue)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount 20 µg
Specification muscle tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: RT°C
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投稿 Jun 19 2015

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse liver, Rat lung)
Gel Running Conditions Reduced Denaturing (gel 12 %)
Loading amount 50 µg
Specification Mouse liver, Rat lung
Blocking step SlimFast as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 18°C
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投稿 May 05 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Feb 27 2015

As I am not experienced in 4-HNE research nor accepted method 4-HNE western blot band densitometry, I must defer to the abReviews and publications that external researches have used ab46545 in. I have listed these below:

abReviews:
http://...

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Thank you for contacting us.

HNE modified protein was used to produce ab46545 (anti-4 hydroxynonenal antibody). It recognizes the specific group in the proteins that had been modified with HNE.

I hope this information is helpful t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"