4-DAMP, Muscarinic receptor antagonist (ab120144)
Key features and details
- Muscarinic receptor antagonist
- CAS Number: 1952-15-4
- Soluble in DMSO to 100 mM and in ethanol to 25 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
4-DAMP, Muscarinic receptor antagonist -
製品の詳細
Muscarinic receptor antagonist -
生理活性の詳細
Muscarinic antagonist. (Ki values for human receptors are 0.57 (M1), 7.3 (M2), 0.37 (M3), 0.72 (M4) and 0.55 nM (M5)).
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CAS 番号
1952-15-4 -
構造式
製品の特性
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体系名
4-Diphenylacetoxy-N-methylpiperidine methiodide -
分子量
451.35 -
分子式
C21H26INO2 -
PubChem 登録番号
3014059 -
保存方法
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM and in ethanol to 25 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
[I-].O=C(OC1CC[N+](C)(C)CC1)C(c2ccccc2)c3ccccc3 -
由来
Synthetic
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研究分野
画像
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2D chemical structure image of ab120144, 4-DAMP, Muscarinic receptor antagonist
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(A) rhAQP5 vector-transfected HSG cells were stimulated with CCh (100 µM) in the presence or absence of 4-DAMP (10 µM), prenzepine (10 µM), and mixture of 4-DAMP and prenzepine (M3R or M1R specific antagonist, respectively).
(B) To monitor hAQP5 trafficking, original GFP pictures in (A) were converted into 'Fire' 3D LUT (lookup tables) setting in ImageJ software and only GFP signal above the fluorescent signal threshold (?80, red or yellow color in converted picture), was captured in (B) as positive GFP signal. Yellow arrows indicate the positive GFP signal on the membrane and green arrows indicates signal in the cytoplasm in HSG cells.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (4)
ab120144 は 4 報の論文で使用されています。
- Tryon SC et al. Differential Regulation of Prelimbic and Thalamic Transmission to the Basolateral Amygdala by Acetylcholine Receptors. J Neurosci 43:722-735 (2023). PubMed: 36535767
- Zhao L et al. Choline Attenuates Cardiac Fibrosis by Inhibiting p38MAPK Signaling Possibly by Acting on M3 Muscarinic Acetylcholine Receptor. Front Pharmacol 10:1386 (2019). PubMed: 31849653
- Muere C et al. Evidence for respiratory neuromodulator interdependence after cholinergic disruption in the ventral respiratory column. Respir Physiol Neurobiol 205:7-15 (2015). PubMed: 25262584
- Lee BH et al. Autoantibodies against muscarinic type 3 receptor in Sjögren's syndrome inhibit aquaporin 5 trafficking. PLoS One 8:e53113 (2013). PubMed: 23382834