Application
Western blot
Sample
Mouse Tissue lysate - whole (B16F10 melanoma)
Loading amount
30 µg
Specification
B16F10 melanoma
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 100ug/ml
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG-HRP
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
Positive control
4-Hydroxynonenal is an a,ß-unsaturated hydroxyalkenal which is produced by lipid peroxidation in cells. 4-HNE has 3 reactive groups: an aldehyde, a double-bond at carbon 2, and a hydroxy group at carbon 4. It is found throughout animal tissues, and in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction, due to the increase in stress events. From wikipedia
=> Truly, under oxidative stress, positive control samples' HNE conc. increases
=> Truly, under oxidative stress, positive control samples' HNE conc. increases
Additional data
Additional Notes
1st lane-control sample, 2nd lane-5gy irradiation-> ROS, 3rd lane-removal of X gene, so it can induce oxidative stress, 4th lane-removal of X gene and 5gy irradiation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Seontae Kim
Verified customer
投稿 Dec 18 2012