製品の概要

  • 製品名
    Anti-3-Nitrotyrosine antibody [2A12]
    3-Nitrotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A12] to 3-Nitrotyrosine
  • 特異性
    This antibody reacts with 3-Nitrotyrosine
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    Ubiquitous species reactivity.
  • 免疫原

    3-Nitrotyrosine conjugated to KLH

  • ポジティブ・コントロール
    • Mouse and rat brain lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab61392 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性
    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
  • 別名
    • Nitrotyrosine antibody

画像

  • All lanes : Anti-3-Nitrotyrosine antibody [2A12] (ab52309) at 1 µg/ml

    Lane 1 : mouse brain lysate
    Lane 2 : rat brain lysate

    Lysates/proteins at 40 µg per lane.


    Observed band size : 25,55,160 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Himori K  et al. Superoxide dismutase/catalase mimetic EUK-134 prevents diaphragm muscle weakness in monocrotalin-induced pulmonary hypertension. PLoS One 12:e0169146 (2017). Read more (PubMed: 28152009) »
  • Chu H  et al. Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress. Sci Rep 7:2252 (2017). WB ; Mouse . Read more (PubMed: 28533545) »

See all 14 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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