製品の概要

  • 製品名Anti-3-Nitrotyrosine antibody [11C2]
    3-Nitrotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [11C2] to 3-Nitrotyrosine
  • 特異性This antibody reacts with free and bound Nitrotyrosine.
  • アプリケーション適用あり: WB, ELISA, ICC, IHC-Pmore details
  • 免疫原

    Chemical/ Small Molecule: Nitrotyrosine conjugated to KLH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500.
ELISA 1/100 - 1/1000.
ICC 1/100 - 1/500.
IHC-P 1/250. PubMed: 19541933

ターゲット情報

  • 関連性Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
  • 別名
    • Nitrotyrosine antibody

Anti-3-Nitrotyrosine antibody [11C2] (ab53232) 使用論文

This product has been referenced in:
  • Guix FX  et al. Modification of ?-secretase by nitrosative stress links neuronal ageing to sporadic Alzheimer's disease. EMBO Mol Med : (2012). WB . Read more (PubMed: 22488900) »
  • McGrath-Morrow S  et al. Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia. Am J Physiol Lung Cell Mol Physiol 296:L565-73 (2009). IHC-P . Read more (PubMed: 19151108) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us. The note on the nitrotyrosine antibody datasheet stating a predicted molecular weight of 1 kDa is incorrect with regards to what one can expect to see in a western blot. Isolated nitrotyrosine may itself be about 1 kDa but ...

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Thank you for your enquiry. I am sorry to confirm that non of our Oxoguanine 8, Nitrotyrosine, and Malondialdehyde antibodies have been tested with half Karnovsky's fixative. Regrettably, this means we therefore cannot confirm and guarantee how well...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"