Anti-Nitrotyrosine 抗体 [11C2] (ab53232)
Key features and details
- Mouse monoclonal [11C2] to Nitrotyrosine
- Suitable for: WB, ELISA, ICC
- Isotype: IgG1
製品の概要
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製品名
Anti-Nitrotyrosine antibody [11C2]
Nitrotyrosine 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [11C2] to Nitrotyrosine -
由来種
Mouse -
特異性
This antibody reacts with free and bound Nitrotyrosine. -
アプリケーション
適用あり: WB, ELISA, ICCmore details -
免疫原
Chemical/ Small Molecule corresponding to 3-Nitrotyrosine conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
11C2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53232の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100 - 1/500.
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ELISA |
1/100 - 1/1000.
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ICC |
1/100 - 1/500.
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特記事項 |
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WB
1/100 - 1/500. |
ELISA
1/100 - 1/1000. |
ICC
1/100 - 1/500. |
ターゲット情報
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関連性
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab53232 は 8 報の論文で使用されています。
- Yan M et al. Trimethylamine N-oxide exacerbates acetaminophen-induced liver injury by interfering with macrophage-mediated liver regeneration. J Cell Physiol 237:897-910 (2022). PubMed: 34459512
- Yan M et al. Cajaninstilbene Acid Ameliorates Acetaminophen-Induced Liver Injury Through Enhancing Sestrin2/AMPK-Mediated Mitochondrial Quality Control. Front Pharmacol 13:824138 (2022). PubMed: 35350766
- Rodriguez J et al. Lack of the brain-specific isoform of apoptosis-inducing factor aggravates cerebral damage in a model of neonatal hypoxia-ischemia. Cell Death Dis 10:3 (2018). PubMed: 30584234
- Yan M et al. Glycycoumarin protects mice against acetaminophen-induced liver injury predominantly via activating sustained autophagy. Br J Pharmacol 175:3747-3757 (2018). PubMed: 30024038
- Guix FX et al. Modification of ?-secretase by nitrosative stress links neuronal ageing to sporadic Alzheimer's disease. EMBO Mol Med : (2012). WB . PubMed: 22488900
- McGrath-Morrow S et al. Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia. Am J Physiol Lung Cell Mol Physiol 296:L565-73 (2009). IHC-P . PubMed: 19151108
- Podowski M et al. Complex integration of matrix, oxidative stress, and apoptosis in genetic emphysema. Am J Pathol 175:84-96 (2009). IHC-P . PubMed: 19541933
- Lagoda G et al. FK506 and sildenafil promote erectile function recovery after cavernous nerve injury through antioxidative mechanisms. J Sex Med 4:908-16 (2007). PubMed: 17627738