製品の概要

  • 製品名
    17 OH Progesterone ELISA Kit
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Control Sera 3 <=8.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Control Sera 3 <=13.8%
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプ
    Competitive
  • 検出感度
    = 0.05 ng/ml
  • 添加回収試験

    98.66 %

  • ステップ
    Multiple steps standard assay
  • 製品の概要

    Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.

     

    A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

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  • Representative Standard Curve using ab108668

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参考文献

ab108668 has not yet been referenced specifically in any publications.

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