17 OH Progesterone ELISA Kit (ab108668)
Key features and details
- Sensitivity: 0.05 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
製品の概要
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製品名
17 OH Progesterone ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Control Sera 3 <=8.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Control Sera 3 <=13.8% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Competitive -
検出感度
= 0.05 ng/ml -
添加回収試験
98.66 %
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ステップ
Multiple steps standard assay -
製品の概要
Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Solution 1 x 50ml 17 OH Progesterone Control 1 x 1ml 17 OH Progesterone Standard 0 – 0 ng/mL 1 x 1ml 17 OH Progesterone Standard 1 – 0.2 ng/mL 1 x 1ml 17 OH Progesterone Standard 2 – 0.6 ng/mL 1 x 1ml 17 OH Progesterone Standard 3 – 2.0 ng/mL 1 x 1ml 17 OH Progesterone Standard 4 – 6.0 ng/mL 1 x 1ml 17 OH Progesterone Standard 5 – 20.0 ng/mL 1 x 1ml 17 OH Progesterone-HRP Conjugate 1 x 22ml Anti-17 OH Progesterone IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究分野
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108668 は 1 報の論文で使用されています。
- Fu Q et al. Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development. Int J Mol Sci 17:N/A (2016). PubMed: 27136540