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Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).
A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
|内容||1 x 96 tests|
|10X Washing Solution||1 x 50ml|
|17 beta Estradiol Control||1 x 0.5ml|
|17 beta Estradiol Standard 0||1 x 1ml|
|17 beta Estradiol Standard 1 (20 pg/mL)||1 x 0.5ml|
|17 beta Estradiol Standard 2 (120 pg/mL)||1 x 0.5ml|
|17 beta Estradiol Standard 3 (300 pg/mL)||1 x 0.5ml|
|17 beta Estradiol Standard 4 (600 pg/mL)||1 x 0.5ml|
|17 beta Estradiol Standard 5 (2,000 pg/mL)||1 x 0.5ml|
|17 beta Estradiol-HRP Conjugate||1 x 22ml|
|Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells)||1 unit|
|Cover foils||1 unit|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
17 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.
Representative Standard Curve using ab108667