17 beta Estradiol ELISA Kit (ab108667)
Key features and details
- Sensitivity: 8.68 pg/ml
- Range: 20 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mammals
製品の概要
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製品名
17 beta Estradiol ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 20 <=9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 <=10% -
サンプルの種類
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Competitive -
検出感度
8.68 pg/ml -
検出範囲
20 pg/ml - 2000 pg/ml -
添加回収試験
101.09 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Mammals -
製品の概要
Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).
A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Washing Solution 1 x 50ml 17 beta Estradiol Control 1 x 0.5ml 17 beta Estradiol Standard 0 1 x 0.5ml 17 beta Estradiol Standard 1 (20 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 2 (120 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 3 (300 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 4 (600 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 5 (2,000 pg/mL) 1 x 0.5ml 17 beta Estradiol-HRP Conjugate 1 x 22ml Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究分野
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab108667 は 45 報の論文で使用されています。
- Iijima H et al. Age-related matrix stiffening epigenetically regulates α-Klotho expression and compromises chondrocyte integrity. Nat Commun 14:18 (2023). PubMed: 36627269
- Wolf DC et al. Sex-specific differences in KCC2 localisation and inhibitory synaptic transmission in the rat hippocampus. Sci Rep 12:3186 (2022). PubMed: 35210456
- Zhong X et al. Sex specificity of pancreatic cancer cachexia phenotypes, mechanisms, and treatment in mice and humans: role of Activin. J Cachexia Sarcopenia Muscle 13:2146-2161 (2022). PubMed: 35510530
- Rodrigues J et al. Oestrogen enforces the integrity of blood vessels in the bone during pregnancy and menopause. Nat Cardiovasc Res 1:918-932 (2022). PubMed: 36531334
- Liu X et al. The AMPK-mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis. Exp Ther Med 21:21 (2021). PubMed: 33235630