Anti-160 kD Neurofilament Medium 抗体 - Neuronal Marker (ab9034)

製品の概要

  • 製品名Anti-160 kD Neurofilament Medium antibody - Neuronal Marker
    160 kD Neurofilament Medium 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 160 kD Neurofilament Medium - Neuronal Marker
  • 特異性Recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa).
  • アプリケーション適用あり: IHC-FoFr, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Cow, Human
    交差が予測される動物種: Mouse, Cat, Pig, Bird, Reptiles
  • 免疫原

    Recombinant C-terminal fragment (Rat).

  • ポジティブ・コントロール
    • Rat spinal cord

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr
IHC-Fr
IHC-P
WB
ICC/IF
  • 追加情報ICC/IF: 1/1000.
    IHC-F: 1/1000.
    IHC-P: 1/1000.
    IHC-Fr: 1/1000.
    WB: 1/5000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
    • 細胞内局在Cytoplasm
    • 参照データベース
    • 別名
      • 150kDa medium antibody
      • NEF3 antibody
      • NEFM antibody
      • Neurofilament 3 antibody
      • NF160 antibody
      • NFM antibody
      see all

    Anti-160 kD Neurofilament Medium antibody - Neuronal Marker 画像

    • The image illustrates the high definition dendritic staining obtained after protease pretreatment. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
    • Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/20000 dilution + whole rat cerebellum homogenate
    • ICC/IF image of ab9034 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9034, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilution

      Lane 1 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.1 µg
      Lane 2 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) 使用論文

    This product has been referenced in:
    • Wang HY  et al. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep 5:17383 (2015). WB ; Mouse . Read more (PubMed: 26619789) »
    • Stegeman S  et al. Loss of Usp9x disrupts cortical architecture, hippocampal development and TGFß-mediated axonogenesis. PLoS One 8:e68287 (2013). Read more (PubMed: 23861879) »

    See all 7 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain tissue)
    Specification Brain tissue
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 12 2011

    We have just received the following information from Novus: "I would guess 10 mg/ml based upon feedback on previous rabbits." I hope this information helps, best of luck with your stainings,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"