Anti-160 kD Neurofilament Medium 抗体 [EP2460] (ab92539)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
IHC-P
ICC/IF
  • 追加情報ICC/IF: 1/100 - 1/250.
    IHC-P: 1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
    IP: 1/10.
    WB: 1/100000 - 1/500000. Predicted molecular weight: 102 kDa.

    Is unsuitable for Flow Cyt.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
    • 細胞内局在Cytoplasm
    • 参照データベース
    • 別名
      • 150kDa medium antibody
      • NEF3 antibody
      • NEFM antibody
      • Neurofilament 3 antibody
      • NF160 antibody
      • NFM antibody
      see all

    Anti-160 kD Neurofilament Medium antibody [EP2460] 画像

    • All lanes : Anti-160 kD Neurofilament Medium antibody [EP2460] (ab92539) at 1/100000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 102 kDa
    • ab92539, at 1/250 dilution, staining 160 kD Neurofilament Medium in formalin-fixed, paraffin-embedded Human normal brain tissue by immunohistochemistry.
    • ab92539, at 1/250 dilution, staining 160 kD Neurofilament Medium in formalin-fixed, paraffin-embedded Human glioma tissue by immunohistochemistry.
    • ab92539, at 1/100 dilution, staining 160 kD Neurofilament Medium in SH-SY-5Y cells by immunofluorescence.
    • ab92539 showing negative staining in Normal breast tissue.

    • ab92539 showing negative staining in Normal colon tissue.

    • ab92539 showing negative staining in Normal tonsil tissue.

    • ab92539 showing negative staining in Normal liver tissue.

    • ab92539 showing negative staining in Hepatocellular carcinoma tissue.

    • ab92539 showing negative staining in Normal colon tissue.

    • ab92539 showing negative staining in Normal liver tissue.

    Anti-160 kD Neurofilament Medium antibody [EP2460] (ab92539) 使用論文

    This product has been referenced in:
    • Foster JW  et al. Low-glucose enhances keratocyte-characteristic phenotype from corneal stromal cells in serum-free conditions. Sci Rep 5:10839 (2015). ICC/IF ; Human . Read more (PubMed: 26039975) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Tissue sections (Human Skin from dermal and epidermal cells)
    Specification Human Skin from dermal and epidermal cells
    Permeabilization Yes - 0.01% Triton X
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Nov 21 2012

    Thank you for contacting us.

    This target should only be expressed in neuronal tissues. Therefore any non-neuronal tissues should serve as a negative control. We have shown negative staining in normal breast tissue, normal colon tissue, nor...

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    Thank you for your phone call.

    I am sorry to hear that both antibodies do not work as expected.


    As for ab92539, I am sending you - as we discussed - the testing discount code for having tried it in IHC-Fr. After submitting you...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"