Anti-160 kD Neurofilament Medium 抗体 (ab72998)
Key features and details
- Chicken polyclonal to 160 kD Neurofilament Medium
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgY
製品の概要
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製品名
Anti-160 kD Neurofilament Medium antibody
160 kD Neurofilament Medium 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to 160 kD Neurofilament Medium -
由来種
Chicken -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat -
免疫原
Recombinant fusion protein containing the extreme C-terminus of rat 160 kD Neurofilament Medium protein expressed in and purified from E. coli.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide -
Concentration information loading...
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精製度
IgY fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72998の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 145 kDa (predicted molecular weight: 96 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 145 kDa (predicted molecular weight: 96 kDa). |
ターゲット情報
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機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
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参照データベース
- Entrez Gene: 24588 Rat
- SwissProt: P12839 Rat
- Unigene: 10971 Rat
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別名
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
画像
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Anti-160 kD Neurofilament Medium antibody (ab72998) at 1/5000 dilution + rat cerebral cortex lysate
Predicted band size: 96 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab72998 は 2 報の論文で使用されています。
- Decourtye L et al. Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation. PLoS One 13:e0193196 (2018). PubMed: 29466413
- Decourtye L et al. IGF-1 Induces GHRH Neuronal Axon Elongation during Early Postnatal Life in Mice. PLoS One 12:e0170083 (2017). PubMed: 28076448