製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 zeta
  • 特異性
    This antibody is specific for 14-3-3 zeta + delta.
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected within aa 200~300 of human 14-3-3 zeta (C-terminal)

  • ポジティブ・コントロール
    • Jurkat cell lysate and human breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 配列類似性
    Belongs to the 14-3-3 family.
  • 翻訳後修飾
    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • 細胞内局在
    Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

画像

  • Anti-14-3-3 zeta antibody (ab37560) at 1/100 dilution + Jurkat cell lysate

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 15 kDa,60 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Staining of formalin-fixed and paraffin-embedded human breast cancer tissue with ab37560 at a 1/50 dilution (using a peroxidase-conjugated secondary antibody, followed by AEC staining).

    These data demonstrate the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

参考文献

ab37560 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab37560.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録