The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Belongs to the 14-3-3 family.
Ser-232 is probably phosphorylated by CK1.
Cytoplasm. In neurons, axonally transported to the nerve terminals.
All lanes : Anti-14-3-3 Tau antibody (ab64991) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells at 5-30ug with no immunizing peptide Lane 2 : Extracts from HeLa cells at 5-30ug with no immunizing peptide Lane 3 : Extracts from HeLa cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size : 28 kDa Observed band size : 28 kDa