製品の概要

  • 製品名Anti-14-3-3 sigma antibody
    14-3-3 sigma 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to 14-3-3 sigma
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human 14-3-3 sigma protein (AAH00329) with a proprietary tag.

  • ポジティブ・コントロール
    • Human ovary cancer tissue lysate. Tagged recombinant Human 14-3-3 sigma protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged 14-3-3 sigma is approximately 0.3ng/ml when used as a capture antibody.

ターゲット情報

  • 機能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • 組織特異性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • 配列類似性Belongs to the 14-3-3 family.
  • 翻訳後修飾Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • 細胞内局在Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody 画像

  • Anti-14-3-3 sigma antibody (ab89405) at 5 µg/ml + Human ovary cancer tissue lysate at 50 µg

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Anti-14-3-3 sigma antibody (ab89405) at 5 µg/ml + Tagged recombinant Human 14-3-3 sigma protein at 0.2 µg

    Predicted band size : 28 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged 14-3-3 sigma is approximately 0.3ng/ml as a capture antibody.

Anti-14-3-3 sigma antibody (ab89405) 使用論文

ab89405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"