製品の概要

  • 製品名Anti-14-3-3 sigma antibody
    14-3-3 sigma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 sigma
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (HYEIANSPEE AISLAKTTFD EAMADLHTLS EDSYKDSTLI MQLLRDNLTL) of Human 14-3-3 sigma (NP_006133).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • 組織特異性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • 配列類似性Belongs to the 14-3-3 family.
  • 翻訳後修飾Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • 細胞内局在Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody 画像

  • Anti-14-3-3 sigma antibody (ab87209) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling 14-3-3 sigma with ab87209 at 2.5µg/ml.

  • Anti-14-3-3 sigma antibody (ab87209) at 2 µg/ml + Sea urchin embryo

    Predicted band size : 28 kDa
    WB Suggested Anti-TNK1 antibody (ab130998) Titration: 1 µg/ml Positive Control: Human pancreatic cancer cell line MiaPaca-2 and Panc-1

Anti-14-3-3 sigma antibody (ab87209) 使用論文

This product has been referenced in:
  • Ding C  et al. Integrin CD11b negatively regulates BCR signalling to maintain autoreactive B cell tolerance. Nat Commun 4:2813 (2013). Mouse . Read more (PubMed: 24264377) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"