製品の概要

  • 製品名Anti-14-3-3 gamma antibody
    14-3-3 gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 gamma
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Cow, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human 14-3-3 gamma.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Skeletal Muscle Tissue; Mouse Brain Tissue; HeLa Whole Cell; NIH 3T3 Whole Cell; K562 Whole Cell.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200. Fix cells using paraformaldehyde and permeabilize with 0.5% Triton X-100/PBS before staining.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

ターゲット情報

  • 機能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 組織特異性Highly expressed in brain, skeletal muscle, and heart.
  • 配列類似性Belongs to the 14-3-3 family.
  • 翻訳後修飾Phosphorylated by various PKC isozymes.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 gamma antibody
    • 14 3 3 protein gamma antibody
    • 14 3 3 protein gamma subtype antibody
    • 14 3 3gamma antibody
    • 14-3-3 protein gamma antibody
    • 1433G_HUMAN antibody
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
    • Ywhag antibody
    see all

Anti-14-3-3 gamma antibody 画像

  • All lanes : Anti-14-3-3 gamma antibody (ab102673) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 150 kDa,40 kDa,60 kDa,90 kDa,>250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ab102673 (1/200) staining 14-3-3 gamma in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • ICC/IF image of ab102673 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab102673, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-14-3-3 gamma antibody (ab102673) 使用論文

ab102673 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Nov 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"