製品の概要

  • 製品名Anti-14-3-3 eta antibody
    14-3-3 eta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 eta
  • 特異性Detects endogenous levels of total 14-3-3 eta protein.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) from an internal region

  • ポジティブ・コントロール
    • Jurkat cell extract HeLa cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab69591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 28 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 組織特異性Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • 配列類似性Belongs to the 14-3-3 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Anti-14-3-3 eta antibody 画像

  • All lanes : Anti-14-3-3 eta antibody (ab69591) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells, using ab69591 at 1/500-1/1000 dilution.

Anti-14-3-3 eta antibody (ab69591) 使用論文

ab69591 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"