製品の概要

  • 製品名Anti-14-3-3 beta + zeta antibody [22-IID8B]
    14-3-3 beta + zeta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [22-IID8B] to 14-3-3 beta + zeta
  • アプリケーション適用あり: WB, ICC, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Lysed transformed amnion cells (AMA) (Human).

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap.


    14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • ポリ/モノモノクローナル
  • クローン名22-IID8B
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 , 30 kDa.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 関連性14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • 細胞内局在Cytoplasmic. Melanosome (fractions from stage I to stage IV)
  • 参照データベース
  • 別名
    • 14 3 3 protein beta antibody
    • 14 3 3 protein beta/alpha antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 zeta antibody
    • HS1 antibody
    • KCIP1 antibody
    • YWHAB antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 beta + zeta antibody [22-IID8B] (ab12347) 使用論文

This product has been referenced in:
  • Kaushal N  et al. The regulation of erythropoiesis by selenium in mice. Antioxid Redox Signal 14:1403-12 (2011). WB ; Mouse . Read more (PubMed: 20969477) »
  • Choi EK  et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem 276:19197-204 (2001). Read more (PubMed: 11278424) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab12347.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"