製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 beta
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 - 141 of 14-3-3 beta (AAH01359).

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates and mouse brain tissue lysate. ICC/IF: HeLa cells. IHC-P: SW480 xenograft and rat cervix tissues.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Predicted molecular weight: 28 kDa.
ICC/IF 1/100 - 1/200.
IHC-P 1/100 - 1/1000.

ターゲット情報

  • 機能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • 配列類似性
    Belongs to the 14-3-3 family.
  • 翻訳後修飾
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • 細胞内局在
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • Epididymis secretory protein Li 1 antibody
    • GW128 antibody
    • HEL S 1 antibody
    • HS1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

画像

  • All lanes : Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 28 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SW480 xenograft labelling 14-3-3 beta with ab97273 at a dilution of 1/500.

  • ab97273, at a 1/200 dilution, staining 14-3-3 beta in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
    Right image is merged with DNA probe.
  • Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution + Mouse brain tissue lysate at 20 µg

    Predicted band size : 28 kDa

    12% SDS PAGE.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cervix labelling 14-3-3 beta with ab97273 at a dilution of 1/500.

  • All lanes : Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : MOLT4 whole cell lysate
    Lane 6 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    12% SDS PAGE

参考文献

This product has been referenced in:
  • Broadbelt KG  et al. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics : (2011). WB ; Human . Read more (PubMed: 21976671) »

See 1 Publication for this product

レビューと Q&A

Thank you for contacting us.

Unfortunately, for ab97273 the immunogenic peptideis also not available for purchase.

I am still waiting for more information about ab43057.

I will keep you informed.
Thank you for your...

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Thank you for contacting us.

I have heard back for ab63635, ab69592:

Unfortunately, for these 2 antibodies the immunogen is not available for sale.

As for ab97273 and ab43057, I am still waiting for a reply and will let y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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