Anti-14-3-3 beta 抗体 (ab97273)
Key features and details
- Rabbit polyclonal to 14-3-3 beta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-14-3-3 beta antibody
14-3-3 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 14-3-3 beta -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 - 141 of 14-3-3 beta (AAH01359).
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ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates and mouse brain tissue lysate. ICC/IF: HeLa cells. IHC-P: SW480 xenograft and rat cervix tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 28 kDa.
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ICC/IF |
1/100 - 1/200.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 28 kDa. |
ICC/IF
1/100 - 1/200. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 7529 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 100153160 Pig
- Entrez Gene: 56011 Rat
- Omim: 601289 Human
- SwissProt: P31946 Human
- SwissProt: Q9CQV8 Mouse
- SwissProt: P35213 Rat
see all -
別名
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
- 1433B_HUMAN antibody
see all
画像
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All lanes : Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SW480 xenograft labelling 14-3-3 beta with ab97273 at a dilution of 1/500.
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ab97273, at a 1/200 dilution, staining 14-3-3 beta in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
Right image is merged with DNA probe. -
Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution + Mouse brain tissue lysate at 20 µg
Predicted band size: 28 kDa12% SDS PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cervix labelling 14-3-3 beta with ab97273 at a dilution of 1/500.
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All lanes : Anti-14-3-3 beta antibody (ab97273) at 1/10000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : MOLT4 whole cell lysate
Lane 6 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
12% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab97273 は 4 報の論文で使用されています。
- Xu C et al. MiR-129-5p sensitization of lung cancer cells to etoposide-induced apoptosis by reducing YWHAB. J Cancer 11:858-866 (2020). PubMed: 31949489
- Li C et al. Low Expression of 14-3-3beta Is Associated With Adverse Survival of Diffuse Large B-Cell Lymphoma Patients. Front Med (Lausanne) 6:237 (2019). PubMed: 31737636
- Wu Q et al. Downregulation of 14-3-3ß inhibits proliferation and migration in osteosarcoma cells. Mol Med Rep 17:2493-2500 (2018). PubMed: 29207109
- Broadbelt KG et al. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics : (2011). WB ; Human . PubMed: 21976671