製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 beta
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-141 of Human 14-3-3 beta

  • ポジティブ・コントロール
    • Human Cerebellum and Skin tissues; A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Predicted molecular weight: 28 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • 配列類似性
    Belongs to the 14-3-3 family.
  • 翻訳後修飾
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • 細胞内局在
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • Epididymis secretory protein Li 1 antibody
    • GW128 antibody
    • HEL S 1 antibody
    • HS1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

画像

  • ab113830, at 5µg/ml, staining 14-3-3 beta in Formalin-fixed, Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
  • ab113830, at 5µg/ml, staining 14-3-3 beta in Formalin-fixed, Paraffin-embedded Human Skin tissue by Immunohistochemistry.
  • All lanes : Anti-14-3-3 beta antibody (ab113830) at 1/10000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : MOLT4 whole cell lysate
    Lane 6 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
  • ab113830 at 1/200 dilution staining 14-3-3 beta in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.

参考文献

ab113830 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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