Anti-14-3-3 alpha + beta 抗体 (ab52995)

製品の概要

  • 製品名
    Anti-14-3-3 alpha + beta antibody
    14-3-3 alpha + beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 14-3-3 alpha + beta
  • 由来種
    Rabbit
  • 特異性
    Broadly reactive with the ~29-32 kDa members of the 14-3-3 family of proteins. With the exception of a single, conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Xenopus laevis, Caenorhabditis elegans
  • 免疫原

    Synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein

  • ポジティブ・コントロール
    • HeLa cell lysate NIH3T3 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use at an assay dependent concentration. The recommended dilution range 0.1-1ug/ml has only been tested in ELISA against the peptide (immunogen). More concentrated antibody might be needed for standard indirect ELISA.

ターゲット情報

  • 機能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • 配列類似性
    Belongs to the 14-3-3 family.
  • 翻訳後修飾
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • 細胞内局在
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 3 3 alpha antibody
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW128 antibody
    • HS 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

プロトコール

参考文献

ab52995 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
ELISA
Sample
Human Tissue sections (cerebrospinal fluid)
Specification
cerebrospinal fluid
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Sandwich (Capture)
Username

Dr. M Schmitz

Verified customer

投稿 Aug 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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