10X RIPA Buffer (ab156034)
製品の概要
-
製品名
10X RIPA Buffer
Lysis Buffer ihc 試薬 製品一覧 -
アプリケーション
適用あり: WB, ELISA, SDS-PAGE, IPmore details -
特記事項
Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
Preparation: Dilute to 1X in deionized water
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at Room Temperature. Store at Room Temperature. -
バッファー
pH: 7.50
Constituents: 0.22% Beta glycerophosphate, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% Sodium lauryl sulfate, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate, 10% Nonylphenol, ethoxylated -
Concentration information loading...
-
研究分野
関連製品
-
Related Buffer
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab156034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Suggested working concentration: 1X
|
|
ELISA |
Use at an assay dependent concentration. Suggested working concentration: 1X
|
|
SDS-PAGE |
Use at an assay dependent concentration. Suggested working concentration: 1X
|
|
IP |
Use at an assay dependent concentration. Suggested working concentration: 1X
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Suggested working concentration: 1X |
ELISA
Use at an assay dependent concentration. Suggested working concentration: 1X |
SDS-PAGE
Use at an assay dependent concentration. Suggested working concentration: 1X |
IP
Use at an assay dependent concentration. Suggested working concentration: 1X |
画像
-
HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.Lane 1: MW marker
Lane 2: Cleared lysateLane 3: Non-soluble
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (67)
ab156034 は 67 報の論文で使用されています。
- Jansens RJJ et al. Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Cell Rep 40:111107 (2022). WB, IP, ICC ; Pig . PubMed: 35858564
- Xie L et al. The HIF-1a/p53/miRNA-34a/Klotho axis in retinal pigment epithelial cells promotes subretinal fibrosis and exacerbates choroidal neovascularization. J Cell Mol Med 25:1700-1711 (2021). PubMed: 33438362
- Foray C et al. Imaging temozolomide-induced changes in the myeloid glioma microenvironment. Theranostics 11:2020-2033 (2021). PubMed: 33500706
- Shati AA & El-Kott AF Acylated ghrelin protects against doxorubicin-induced nephropathy by activating silent information regulator 1. Basic Clin Pharmacol Toxicol 128:805-821 (2021). PubMed: 33547742
- Chen Q et al. Rho-kinase inhibitor hydroxyfasudil protects against HIV-1 Tat-induced dysfunction of tight junction and neprilysin/Aß transfer receptor expression in mouse brain microvessels. Mol Cell Biochem 476:2159-2170 (2021). PubMed: 33548010