製品の概要

  • 製品名10x EDTA Buffer pH 8.0
    EDTA buffer ihc 試薬 製品一覧
  • アプリケーション適用あり: IHC-Pmore details
  • 特記事項Recommended Procedure: Microwave
    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:10 with deionized water.
    3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
    4. Keep the slides warm by heating for 10min at LOW power.
    5. Let coplin jar sit in microwave for at least 20min.
    6. Remove and rinse with buffer.
    7. Proceed with staining.

製品の特性

  • 関連性EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
  • 別名
    • Ethylenediaminetetraacetic acid buffer

アプリケーション

Our Abpromise guarantee covers the use of ab64216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.

プロトコール

10x EDTA Buffer pH 8.0 (ab64216) 使用論文

ab64216 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and patience.

I have checked with my colleagues and the concentration is 10 mM EDTA. We will update the datasheet accordingly very soon.

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Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" ca...

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ab64216 is supplied with a 10 mM solution of EDTA which is a 10X stock solution. The working concentration is 1mM EDTA. I apologize for any confusion. Please do not hesitate to contact me with additional questions or concerns.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"