10kD Spin Column (ab93349)
製品の概要
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製品名
10kD Spin Column -
製品の概要
The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration or deproteinization of biological samples.
They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g (5-10 mins). The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.
Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 1997 10K Spin Column. 1997-25 is the same size as the 25 unit size of ab93349.
If working with cell or tissue lysates, we generally recommend the TCA Sample Deproteinization Kit or using a PCA/KOH Deproteinization protocol. If it is critical to use a spin column with a cell or tissue lysate then, to avoid blocking the filter, first centrifuge the lysate samples at ~14,000 rpm for 10-20 mins to pellet debris, and then use the resulting supernatant with the spin column.
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アプリケーション
適用あり: Purificationmore details
製品の特性
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保存方法
Store at room temperature. Please refer to protocols. -
内容 25 units 25 units Concentrator cup: Polycarbonate 25 units 25 units Filtrate vessel: Polypropylene 25 units 25 units Membrane: 10,000 MWCO Polyethersulfone 25 units 25 units -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Purification |
Use at an assay dependent concentration.
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特記事項 |
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Purification
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (27)
ab93349 は 27 報の論文で使用されています。
- Gong J et al. The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonates. JCI Insight 6:N/A (2021). PubMed: 33497360
- Nayak G et al. Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light Sensing. Cell Rep 30:672-686.e8 (2020). PubMed: 31968245
- Ortiz-Masiá D et al. Succinate Activates EMT in Intestinal Epithelial Cells through SUCNR1: A Novel Protagonist in Fistula Development. Cells 9:N/A (2020). PubMed: 32365557
- Feng D et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32629939
- Sharma I et al. Myo-inositol Oxygenase (MIOX) Overexpression Drives the Progression of Renal Tubulointerstitial Injury in Diabetes. Diabetes 69:1248-1263 (2020). PubMed: 32169892